List of Changes to the Standard Operating Procedures (SOPs)
The following is a summary of
the changes to the field and
lab SOPs. Please contact
for further information.
||Adds proficiency evaluation criteria for the Lake Vegetation Index (LVI) procedure. Adds recommended
training procedures and proficiency evaluation checklists for conducting Stream Condition Index (SCI) sampling and
stream habitat assessments to the Appendix.
||Clarifies language in the Field Quality Control SOP concerning the collection of blank samples for certain applications.
||Clarifies and updates the sample preservation and holding time tables, which include new EPA requirements and
additional information for analytes required for certain DEP programs.
||Adds a discussion on composite sample types and a procedure for sulfide sampling. Updates the procedure for cyanide sampling.
||Adds a procedure to collect grab samples with prepreserved containers.
||Updates the Groundwater sampling SOP to: add special procedures for purging and sampling deep, large-volume,
high-recharge wells; to clarify groundwater sample filtration procedures; to allow the collection of water samples
directly through the peristaltic pump-head assembly for extractable organic compounds; and, to address other miscellaneous issues.
||Adds the option for the collection of water samples directly through the peristaltic pump-head assembly for extractable organic compounds.
||Adds a minimum wait period for sampling for biorecon or SCI after flood events and clarifies the criteria for
determining flood conditions. Revises the definition of productive and minor habitats for biorecon and SCI
sampling. Makes minor revisions to the SCI sampling procedure. Revises the Qualitative Periphyton Sampling
Procedure. Adds the Rapid Periphyton Survey (RPS) and the Lake Vegetation Index (LVI) sampling procedures. Adds the
RPS and LVI field sheets.
||Changes the calibration acceptance criterion (verification with NIST-traceable thermometer) to ± 0.5 oC.
||Updates the Dissolved Oxygen (DO) field testing procedure to allow use of the Luminescent DO sensor technology as an approved device.
||Clarifies the procedure for observing the Secchi disk from a boat.
||Deleted (Field Oxidation Reduction Potential)
||Deleted (Field Sulfite)
||Deleted (Field Sediment Oxygen Demand)
||Deleted (Field Dissolved Gases)
||Adds a definition of a typical stream transect and an example of how to observe stream velocity. Updates the
stream habitat assessment procedure and the stream habitat assessment field sheet.
||Selected forms are revised, consolidated or removed from various SOPs.
||References listed at the end of many of the SOPs have been deleted (if outdated) or moved into the text of the SOPs in appropriate sections.
||Adds documentation requirements for the
||Adds optional quality control procedures
for the LVI determination.
||Updates the Stream Condition Index (SCI)
and the BioRecon (Rapid Bioassessment Method) Index
calculation and scoring procedures based on the results
of a recent recalibration analysis. Adds the LVI scoring
procedure, based on the report “Assessing the
Biological Condition of Florida Lakes: Development
of the Lake Vegetation Index (LVI)", Fore, 2007. Deletes
the LCI scoring and categories table. Updates the
reference to the revised report, "Development and
Testing of Biomonitoring Tools for Macroinvertebrates in
Florida Streams" (Fore, 2007). Adds a reference to an
important SCI training and reference document, “Sampling
and Use of the Stream Condition Index (SCI) for
Assessing Flowing Waters: A Primer” (FDEP,
Environmental Assessment Section, Bureau of
Laboratories, DEP/EA/002/07, June 2007).