List of Changes to the Standard Operating Procedures (SOPs)
The following is a summary of
the changes to the field and
lab SOPs. Please contact
for further information.
||Adds proficiency evaluation criteria for
the Lake Vegetation Index (LVI) procedure. Adds
recommended training procedures and proficiency
evaluation checklists for conducting Stream Condition
Index (SCI) sampling and stream habitat assessments to
||Clarifies language in the Field Quality
Control SOP concerning the collection of blank samples
for certain applications.
||Clarifies and updates the sample
preservation and holding time tables, which include new
EPA requirements and additional information for analytes
required for certain DEP programs.
||Adds a discussion on composite sample
types and a procedure for sulfide sampling. Updates the
procedure for cyanide sampling.
||Adds a procedure to collect grab samples
with prepreserved containers.
||Updates the Groundwater sampling SOP to:
add special procedures for purging and sampling deep,
large-volume, high-recharge wells; to clarify
groundwater sample filtration procedures; to allow the
collection of water samples directly through the
peristaltic pump-head assembly for extractable organic
compounds; and, to address other miscellaneous issues.
||Adds the option for the collection of
water samples directly through the peristaltic pump-head
assembly for extractable organic compounds.
||Adds a minimum wait period for sampling
for biorecon or SCI after flood events and clarifies the
criteria for determining flood conditions. Revises the
definition of productive and minor habitats for biorecon
and SCI sampling. Makes minor revisions to the SCI
sampling procedure. Revises the Qualitative Periphyton
Sampling Procedure. Adds the Rapid Periphyton Survey
(RPS) and the Lake Vegetation Index (LVI) sampling
procedures. Adds the RPS and LVI field sheets.
||Changes the calibration acceptance
criterion (verification with NIST-traceable thermometer)
to ± 0.5 oC.
||Updates the Dissolved Oxygen (DO) field
testing procedure to allow use of the Luminescent DO
sensor technology as an approved device.
||Clarifies the procedure for observing the
Secchi disk from a boat.
||Deleted (Field Oxidation Reduction
||Deleted (Field Sulfite)
||Deleted (Field Sediment Oxygen Demand)
||Deleted (Field Dissolved Gases)
||Adds a definition of a typical stream
transect and an example of how to observe stream
velocity. Updates the stream habitat assessment
procedure and the stream habitat assessment field sheet.
||Selected forms are revised, consolidated
or removed from various SOPs.
||References listed at the end of many of
the SOPs have been deleted (if outdated) or moved into
the text of the SOPs in appropriate sections.
||Adds documentation requirements for the
||Adds optional quality control procedures
for the LVI determination.
||Updates the Stream Condition Index (SCI)
and the BioRecon (Rapid Bioassessment Method) Index
calculation and scoring procedures based on the results
of a recent recalibration analysis. Adds the LVI scoring
procedure, based on the report “Assessing the
Biological Condition of Florida Lakes: Development of
the Lake Vegetation Index (LVI)", Fore, 2007. Deletes
the LCI scoring and categories table. Updates the
reference to the revised report, "Development and
Testing of Biomonitoring Tools for Macroinvertebrates in
Florida Streams" (Fore, 2007). Adds a reference to an
important SCI training and reference document, “Sampling
and Use of the Stream Condition Index (SCI) for
Assessing Flowing Waters: A Primer” (FDEP,
Environmental Assessment Section, Bureau of
Laboratories, DEP/EA/002/07, June 2007).
Water Quality Standards Program
Florida Department of Environmental Protection
2600 Blair Stone Road - M.S. 6511
Tallahassee, FL 32399