The North Fork St. Lucie River Aquatic Preserve (NFSLRAP) is located in southeast Florida, approximately 40 miles northwest of West Palm Beach.

The preserve is bounded on the north by Midway Road in White City. The southern preserve boundary extends from Coconut Point in Stuart to Jenkins Point in Palm City just west of the Roosevelt Bridge in Martin County. The eastern and western boundaries encompass the state-owned sovereign submerged lands occurring below the mean high water line to which the state holds title. The preserve is approximately 16 miles long through the natural riverbends.

Five bridges currently cross the aquatic preserve.

1. Midway Road at the northern boundary.
2. Prima Vista Boulevard, approximately four miles south of the northern boundary.
3. Port St. Lucie Boulevard, approximately 10 miles south of the northern boundary.
4. Mapp Road (over the C-23 Canal at the southwest boundary).
5. Murphy Road (over the C-23 Canal at the southwest boundary).

The headquarters for the NFSLRAP is the Southeast Florida Aquatic Preserves Field Office, located in Ft. Pierce, Florida. The office is on public land managed by the Savannas Preserve State Park located at the meeting of Five Mile and Ten Mile creeks. The headquarters is approximately two miles north of the aquatic preserve boundary at Midway Road.